![]() ![]() The Akadeum microbubble protocol enables quick and easy B cell enrichment without requiring additional equipment or expensive consumables like magnets or columns. Fast Processing Įxperience the Benefits of a Microbubble Workflow An optional centrifugation step pellets the B cells, and the microbubbles, microbubble-bound cells, and supernatant are easily aspirated away leaving the untouched and enriched B cells happy, healthy, and ready for downstream use. Once bound, they quickly and gently float the undesired cells to the surface. Next, the microbubbles are mixed into the sample where the seek out and bind to the unwanted cells. How it Works: First, Akadeum’s biotinylated antibody cocktail is added to the sample to label the contaminating cells. Ideal for the enrichment of delicate immune cells, separation occurs directly in the sample container without exposing cells to harsh chemicals or harmful shear forces. Using Akadeum’s Mouse B Cell Isolation Kit to study B cell differentiation into plasma cells (University of Pennsylvania)Īkadeum’s Mouse B Cell Isolation Kit delivers a high-purity population of healthy, viable mouse B cells using a protocol that is fast, easy, and exceptionally gentle on cells. ![]() Using Akadeum’s Mouse B Cell Isolation Kit to study targeted delivery of anti-tumor DNA vaccines to B cells as a novel alternative to chemotherapy (University of Wisconsin)ĭifferentiation Potential Maintained Through Microbubble-Based Separation Using Akadeum’s Mouse B Cell Isolation Kit to study autoreactive B cell activation and differentiation (University of California, San Francisco)Īntigen Presenting Capacity Retained After B Cell Enrichment with Microbubbles See how different labs around the country have used Akadeum’s Mouse B Cell Isolation Kit to enrich for unaltered B cells that maintain their native physiological state.īCR Isotype Distribution Remains Unchanged Following Microbubble Isolation This novel, non-invasive cell isolation method does not disturb BCRs or skew the isolated populations. Using Akadeum’s exceptionally gentle, microbubble-based approach to separation allows for the enriched B cells to retain their activation and differentiation potential. Mouse B Cell Enrichment with Microbubbles Maintains Cell Health & Physiology Shop Mouse B Cell Enrichment Microbubbles > The end result is a pure population of physiologically unaltered B cells that are ready for downstream use. This enables quick and easy sample preparation without requiring additional equipment or expensive consumables like magnets or columns while eliminating the need for pour-offs and tube transfers. With Akadeum’s microbubble workflow, the entire separation process occurs directly in the sample container without exposing cells to harsh chemicals or harmful shear forces. These hybridomas are then screened to identify the high-producing clones that yield antibodies with superior antigen specificity, and these selected clones are then expanded to increase production.Īkadeum’s Mouse B Cell Isolation Kit can be used to optimize the purification stage, enriching splenocyte samples for highly viable B cells for use in generating hybridomas.įigure 1: Enhanced Hybridoma Workflow Using Akadeum’s Mouse B Cell Isolation Kit Hybridoma technology involves the fusion of cells from immunized hosts with immortalized myeloma cells to create a hybridoma. Generally speaking, the hybridoma workflow is laborious and resource-intensive, and has not seen many improvements or optimizations since its inception. Hybridoma technology, first discovered in 1975, has quickly become the preferable method of mAb production 1. Monoclonal antibodies (mAbs) are critical biomolecules with applications that span research, diagnostics, therapeutics, and beyond. Integrating Microbubbles into Hybridoma Workflows Fast, Easy, and Effective B Cell EnrichmentĪkadeum’s Mouse B Cell Isolation Kit delivers a highly enriched population of healthy, viable B cells for use in hybridoma-based monoclonal antibody workflows.Īkadeum’s Mouse B Cell Isolation Kit delivers a highly enriched population of healthy, viable B cells using a protocol that is fast, easy, and exceptionally gentle on delicate immune cells.Reduce Time and Resources Spent on Screening.Increase Frequency of Antibody-Producing Hybridomas.Stop losing your precious cells to outdated processing techniques.Īkadeum’s revolutionary microbubble approach to B cell isolation delivers highly enriched B cells while maintaining cellular health and physiology. Akadeum’s Microbubbles offer a gentle approach to B cell enrichment that can enhance monoclonal antibody production by improving hybridoma workflows.Īkadeum’s patented Buoyancy Activated Cell Sorting (BACS™) technology can eliminate wasted time and resources by increasing the frequency of useful hybridomas that are generated. ![]()
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